Sterilization and Culture methods detailed
Sterilization and Culture methods detailed
Introduction sterilization
Definition and Methods
Physical Methods - Red Heat
Hot Air Oven
Moist heat and auto clave
Pasteurization
IR radiation
Non IR
Filtration
5 types of Filtration
chemical method- two types of gases
Liquid stage
4 types of Liquid chemicals
Applications
Introduction Culture methods
culture media definition and ingredients
types of media
physical media
semi solid media
solid media
chemical media 3 types
non synthetic media
special media 3 types
Enriched media
selective media
differential media
anaerobic media
Transport media
Antibiotic media
conclusion
Two major contributions to the art of sterilization came in the 1860’s
when the French chemist and microbiologist Louis Pasteur
wrote extensively on how germs cause disease and the English physician, Joseph Lister,
developed a technique that used carbolic acid as a spray to disinfect instruments and so on. (refer video slides all full info)
The first liquid artificial culture medium was created by Louis Pasteur in 1860
Previously bacterial growth on daily materials such as some foods had been observed
Louis Pasteur was a French chemist and microbiologist renowned for his discoveries of the principles of vaccination microbial fermentation and pasteurization
The German physician Robert Koch (December 11 1843 — May 27 1910) is considered the father of modern bacteriology for his work demonstrating that specific microbes are responsible for causing specific diseases
Koch discovered the life cycle of the bacteria responsible for anthrax and identified the bacteria that cause tuberculosis and cholera
Culture media
A microbiological culture medium is a substance that encourages the growth support and survival of microorganisms
Culture media contains nutrients growth promoting factors energy sources buffer salts minerals metals and gelling agents (for solid media)
Different types of culture media are typically divided based on the physical state of the media
Liquid culture media
commonly called broth Solid and semi-solid culture media commonly called agar. (refer video slides all full info)
sterilization and culture media concepts
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What you will learn
- Introduction sterilization
- Definition and Methods
- Physical Methods - Red Heat
Rating: 3.75
Level: All Levels
Duration: 3 hours
Instructor: Sowjanya Chollangi
Courses By: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
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