Build Strong Foundations- PCR, Multiplex PCR, DNA Sequencing




Build Strong Foundations- PCR, Multiplex PCR, DNA Sequencing

Do you intent to refresh your PCR knowledge and also learn new, unaware PCR tips?  Small steps you take could lead to success in PCR

Are you planning to upgrade your PCR skills to higher  level with new possibilities? increase per PCR output

Do you want to take advantage of PCR skills for fluorescent DNA sequencing application?  not handing over your precious samples completely

(Master sample processing upto capillary electrophoresis step, if  electrophoresis is done elsewhere outside your lab)

How to visualize your gene sequence graphs after Sanger sequencing? available free to download sequence visualization tools

Curious how PCR failures are handled to resolve the issue? Step-by-Step detailed methodology given

Look no further and enrol now and see for yourself.

The course is for

  • Biology, Biotechnology, Molecular biology  (undergraduate and Master's) students

  • Undergraduate interns at PCR laboratories, research labs

  • Genetic/Molecular/Medical laboratory professionals


    What motivated me to  construct the course

PCR is  the foundation of molecular biology which led to development of other molecular methods.

Felt disconnect between  PCR practical knowledge and traditional classroom instruction.

The tips and PCR improvements learned through my trial and error methods during my PCR journey

The learning format include 

  1. Complete one-stop shop- Primers to PCR gel analysis 

  2. Study review questions, assignments and practice tests

  3. Images from real-bench work, examples and web-based demonstrations for easy following.

  4. Example of using well established molecular genetic resource to retrieve gene information

  5. Learn the ten steps for confident PCR

  6. Acquire skills  in researching and gathering genomic data from web-based Databases(NCBI as example),  processing the gene sequence to FASTA format for primer design or sequence analysis( NCBI primer-BLAST tool)

I guarantee my personal attention to your queries during your learning experience and enthusiastic about ensuring a  good learning experience for you now and in future. You could also add the course to your wish list for future enrollment.

Thank you and see you soon after enrollment.

Biju Joseph






Become a Master < Learn PCR/ mPCR tips; familiarize yourself with downloadable sequence view tools;Solve PCR failures>

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What you will learn
  • PCR primers - Calculations, Dilutions, Design using NCBI -BLAST tool within minutes (see the demonstration for how to........)
  • Learn about "other PCR flavors" for inconsistent target gene amplification by standard PCR
  • Confident PCR results always- 10 rule cheatsheet

Rating: 3.5

Level: Beginner Level

Duration: 3 hours

Instructor: Biju Joseph Ph.D.


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